Top latest Five attune flow cytometer Urban news
Top latest Five attune flow cytometer Urban news
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Data-enriched characterization of mobile populations. Morphological facts from images can incorporate on the richness of flow cytometry info. Such as, the figure shows an usually standard apoptosis assay utilizing Annexin V and PI, incorporating mobile imaging to characterize cells in Just about every populace to reveal morphologically distinct attributes. These insights couldn't are attained from multiplex staining by yourself.
As samples are acquired to the Attune CytPix Flow Cytometer, the superior-velocity brightfield digicam captures and shops images of detected gatherings in a charge as much as six,000 images per 2nd, depending upon the flow level and impression size. For greater flexibility, Attune Cytometric Application enables you to regulate the graphic seize frequency as needed and choose a certain gate for picture seize, if necessary.
Combine acoustic concentrating technology with high-parameter Examination for adaptable spectral unmixing and traditional workflows.
Learn new insights into your experimental facts by visualizing populations using combined fluorescence and morphology data. Our computer software interface wizards help downsampling, concatenation (merged data files) and dimensionality instruments that are created into the program for an intuitive, streamlined facts Examination working experience.
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Additionally, it can be illuminating to discover the number of doublets slip via standard singlet gates, and terrific to validate the status of sudden double-good activities in my Investigation."
Optimizing gating strategy. Even robust guide singlet gating is error-susceptible and stays a subjective choice stage in Virtually all flow cytometry assays. Imaging can be used to verify and alter gates to incorporate only single cells of fascination.
Our experts will structure panels to suit your needs as often times you'll need—cost-free, no products invest in required.
The Attune NxT cytometer would be the trustworthy workhorse instrument your lab can rely on for constant and reliable general performance. The Attune CytPix product incorporates a high-speed brightfield camera and AI-driven picture Investigation software program, with new upgrades like device Understanding tools and an intuitive consumer interface, enabling Improved data precision, precision, and more rapidly insights into sample biology.
Customers can look at function photos in real-time to substantiate sample high-quality, enhance protocols, and history the morphology of each and every cell populace identified for Investigation. Imaging seize also Advantages from acoustic focusing, which really helps to position the cells to ensure a sharp, centered picture is received.
Seem waves enable specific cell alignment, leading to exceptional data good quality and ~10X faster sample flow charges.
We Formerly demonstrated the strength of imaging Motor vehicle-T/Ramos mobile interactions. Let’s check out just the population of finest fascination, the double positive occasions, To find out more. We will now use extended morphological parameters (below, circularity and skewness of intensity) to additional look at the features of those populations and refine gating on these situations, expanding information robustness.
Created-in dimensionality reduction equipment improved resolution into populations. Fluorescently gated populations again-gated onto UMAP plot to verify anticipated populace resolution in lysed complete read more human blood. Person can see events from Every cluster for visual affirmation of mobile phenotypes.
Versatile payment approach from Agilent Economic Remedies help you get the crucial instrumentation you will need for speedy job, or to increase your lab.
At the heart of every Attune Flow Cytometer lies our innovative acoustic focusing engineering, which utilizes sound waves to meticulously align cells within the flow cell. This ground breaking method surpasses traditional hydrodynamic techniques, resulting in more quickly processing speeds, Outstanding details high-quality, and greater sample throughput.
The Attune Xenith cytometer is usually a significant-parameter spectral analyzer, giving each spectral unmixing and standard flow capabilities for thorough and functional cell Assessment.
Agilent Benefit Promise assures you no less than 10 years of instrument use in the day you buy. If not, We're going to credit score you with the residual worth of the procedure toward an upgraded model.
What seemed to be a homogeneous population is definitely much more varied—an insight that ought to be deemed when interpreting final results.